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| Mezzalira,Alceu; Viera,Arnaldo Diniz; Barbieri,Dilmar Paulo; Machado,Mariana Fernandes; Thaler Neto,André; Bernadi,Mari Lourdes; Silva,Carlos Antônio Mondino; Rubin,Mara Iolanda Batistella. |
| In this study, the influence of cytochalasin B on the survival and development after vitrification of oocytes and in vitro produced (IVP) bovine blastocysts was evaluated. In the Experiment I, 956 oocytes were matured for 22h and were immediately vitrified (Vitri treatment) or exposed for 15-20 minutes, to 7.5µg/ml (CB7.5Vitri treatment) or 45µg/ml (CB45Vitri treatment) cytochalasin B solutions, before vitrification. After 30 seconds of exposure to SV1 [400µl TCM-Hepes with 10% fetal serum (SF), 50µl ethylene glycol (EG) and 50µl DMSO], and 20 seconds to SV2 (300µl trehalose 1,0M + 20% SF, 100µl EG and 100µl DMSO) solutions, the oocytes were vitrified in Open Pulled Straws (OPS). The rewarming was performed at 37-38ºC in two steps of 5 minutes each, into... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Cytochalasin B; Embryos. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000500008 |
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